Table 4.
The effects of different cell culturing methods on BMMSC cell population and CXCR4 expression level
| Characteristic | Day 0 | Day 3 | Day 6 | Day 9 | |||
|---|---|---|---|---|---|---|---|
| PC | MC | PC | MC | MC Bead‐T | MC Bead‐T | ||
| A division | 82.5 ± 1.4% | 78.5 ± 7.3% | 90.5 ± 2.8%† | 64.2 ± 1.0% | 78.8 ± 1.2%‡ | 83.9 ± 0.3%‡ | 80.0 ± 1.8% |
| B division | 13.3 ± 1.3% | 18.4 ± 6.1% | 7.7 ± 2.7%* | 33.6 ± 1.3% | 21.2 ± 0.7%‡ | 8.5 ± 1.0%‡ | 14.1 ± 0.6% |
| C3 division | 81.6 ± 1.4% | 85.1 ± 1.0% | 95.6 ± 0.1%‡ | 91.6 ± 6.5% | 94.1 ± 4.0% | 74.0 ± 1.6%* | 82.8 ± 3.2% |
| CXCR4+ | 99.2 ± 0.2% | 86.9 ± 0.5% | 95.4 ± 6.4%‡ | 44.1 ± 3.4% | 44.0 ± 3.0% | 95.7 ± 1.2%‡ | 79.4 ± 2.1% |
| X‐Mean | 47.4 ± 0.6 | 12.9 ± 1.9 | 44.5 ± 3.7* | 5.8 ± 0.2 | 5.81 ± 0.1 | 41.1 ± 1.0‡ | 7.4 ± 0.6 |
Cell population and CXCR4 expression level of BMMSC from MC or MC Bead‐T was compared with that from PC for t‐test analysis.
X‐Mean: mean fluorescence (FITC) intensity of CXCR4‐positive cells.
*P < 0.05, †P < 0.01, ‡P < 0.005.