Table 1.
Primers of housekeeping genes (GAPDH and β‐actin) and related growth factor genes designed for semi‐quantitative PCR
| mRNA | Primer pairs |
|---|---|
| GAPDH (233 bp) |
Forward GGTGAAGGTCGGTGTGAACG Reverse CTCGCTCCTGGAAGATGGTG |
| β‐ACTIN (266 bp) |
Forward GTCCCTCACCCTCCCAAAAG Reverse GCTGCCTCAACACCTCAACCC |
| VEGFA (106 bp) |
Forward CTGCTGTGGACTTGTGTTGG Reverse AAAGGACTTCGGCCTCTCTC |
| VEGFB (128 bp) |
Forward GCAACACCAAGTCCGAATG Reverse CTGGCTTCACAGCACTCTCC |
| TGF‐β1(113 bp) |
Forward TGGAGCCTGGACACACAGTA Reverse TAGTAGACGATGGGCAGTGG |
| IGF‐1(171 bp) |
Forward CTGCTTGCTCACCTTCACC Reverse TCATCCACAATGCCTGTCTG |
| HIF‐1α (129 bp) |
Forward TGAACATCAAGTCAGCAACG Reverse CACAAATCAGCACCAAGCAC |
| EGF‐1(112 bp) |
Forward GCTCTTCTGGGTTCAGGACA Reverse AGACAAACTGTGCCGTGCTT |
| VE‐ca (102 bp) |
Forward CATCGCAGAGTCCCTCAGTT Reverse TCAGCCAGCATCTTGAACCT |
| Vcl(122 bp) |
Forward GTCTGTGAGCGAATCCCAAC Reverse ATGAACCAGCATCTCTGTGG |
| PGF(107 bp) |
Forward CCGATAAAGACAGCCAACATC Reverse CATTCACAGAGCACATCCTGA |
| FGF‐1(124 bp) |
Forward CCACAGCCCAGCAGTTATC Reverse CTCCTACGCCCACTCTTCAG |
| FGF‐2(138 bp) |
Forward AGGAAGATGGACGGCTGCT Reverse GCCCAGTTCGTTTCAGTGC |
| BMP‐2(106 bp) |
Forward TGAGGATTAGCAGGTCTTTGC Reverse CGTTTGTGGAGCGGATGT |
| BMP‐4(103 bp) |
Forward TCTTCAACCTCAGCAGCATC Reverse AAGCCCTGTTCCCAGTCAG |
| BMP‐5(100 bp) |
Forward CACCAGGGAAACAAGCATCT Reverse CTCACCAAATACTCCGACTCCT |
| BMP‐6(124 bp) |
Forward AGGTTCCATTCCCAGCAAG Reverse TCACACCACCGAGAGTCAAC |
| BMP‐7(101 bp) |
Forward TCCTGCATCCACACAAAGAA Reverse TTCCAGGGACACAGACATGA |