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. 2010 Jun 29;43(4):354–364. doi: 10.1111/j.1365-2184.2010.00684.x

Figure 2.

Figure 2

 Visualization of DNA damage by detection of γH2A.X in oocytes and embryos treated with curcumin. Chromatin shown in red, γH2A.X shown in green. a, b and c – merge of chromatin and γH2A.X staining; a′, b′ and c′γH2A.X only. (A) Oocytes treated with curcumin at GV stage and cultured during time necessary for finishing I meiosis. a. CTR – control, b. 40 μm curcumin, c. 300 nm doxorubicin. (B) Embryos treated with curcumin at two‐cell stage and cultured during time necessary to undergo second and subsequent mitosis. a. CTR – control eight‐cell embryo (nuclei of five cells visible, rest of cells are in different focal planes), b. four‐cell embryo treated with 40 μm curcumin, c. two‐cell embryo F treated with 300 nm doxorubicin (non dividing); arrow points to polar body. Approximately 50 oocytes or embryos in each group. Curcumin did not induce DNA damage. In oocytes, γH2A.X was detected only in the polar body, designated for degradation, but not in chromatin area of the egg (A). In embryos, no significant difference was observed between control and curcumin‐treated embryos (B). Scale bar: 20 μm.