Figure 4.

 GV‐arrested oocytes pre‐incubated with curcumin. Ability to finish I meiotic division of GV‐arrested control oocytes and GV‐arrested oocytes pre‐incubated 6 h with 30 and 50 μm curcumin after replacement with fresh medium. (A) Percentage of oocytes, which extruded I polar body (Ipb). Each group contained ∼150 oocytes. GVBD – oocytes started meiotic resumption but did not extrude Ipb. (B) Chromatin shown in red, α‐tubulin shown in green. Control oocytes and oocytes pre‐incubated with 50 μm curcumin – after replacement with fresh medium and cultured for time necessary for completion of I meiosis. Scale bar: 20 μm. Pre‐incubation with 50 μm curcumin impaired cytokinesis and resulted in decrease in ability to finish first meiotic division. Polar body was still connected to oocyte (arrow).