Figure 5.

 Electron microscopy analysis of tubulin polymerization. Electron microscopy analysis of tubulin polymerized in the absence (A, C) and in presence of curcumin (B, D). Tubulin polymerization performed in presence (A and B) and in absence of taxol (C and D). Microtubules assembled in presence of taxol were stable and did not depolymerize upon dilution (A). Curcumin did not affect number of formed microtubules nor globular tubulin oligomers (B). Microtubules formed in absence of taxol disassemble into oligomers upon dilution (C). Curcumin neither stabilized microtubules nor affected tubulin oligomerization (D). Microtubules and tubulin oligomers in presence and absence of curcumin looked the same.