Figure 6.

 Western blot analysis of tubulin acetylation status in oocytes treated with curcumin. Western blot analysis of acetylated α‐tubulin and α‐tubulin. Oocytes were treated with 30 and 50 μm concentration of curcumin. (A) Oocytes treated at GV stage and cultured for time necessary for formation of meiotic spindle (about 6 h). Positive control, oocytes treated with TSA – commonly used inhibitor of deacetylases. (B) Oocytes treated at GV stage and cultured for time necessary to finish I meiotic division (about 11 h). 30, 50–30 and 50 μm concentration of curcumin. AcT, α‐acetylated tubulin; T, α‐tubulin. Each lane contained lysate from 25 oocytes. Representative picture from one of three independent experiments.