Table 1.

Primer sequences, PCR cycle number and expected product sizes

Gene	Primer forward 5′–3′	Primer reverse 5′–3′	Cycles	Product size (base pair)
GAPDH	GGGCTGCTTTTAACTCTGGT	TGGCAGGTTTTTCTAGACGG	32–35	710
p53	CCCCTCTGAGTCAGGAAACA	TCATCTGGACCTGGGTCTTC	35	151
p16	TGCCTTTTCACTGTGTTGGA	AGCTTTGGTTCTGCCATTTG	35	196
RB	ACACAACCCAGCAGTTCGAT	CTTTGAGCAACATGGGAGGT	35	187
H‐RAS	GTTGGACATCCTGGATACCG	CTTCACCCGTTTGATCTGCT	32	252