Figure 4.

MiR‐330‐3p mimics abrogated the function of RASSF1A knockdown in non‐small‐cell lung cancer (NSCLC) cell lines. A, Cell proliferation assay was evaluated by CCK‐8 assay. In A549 and NCI‐H23 cell lines, miR‐330‐3p inhibitor suppressed cell proliferation, whereas miR‐330‐3p mimics promoted it. Co‐transfection of mimics and RASSF1A inhibited this promotion. B,Transfection of NSCLC cell lines with miR‐330‐3p resulted in G0/G1 phase arrest by FACS flow cytometry. C, The apoptotic cells in the mimics group, inhibitor group and mimics + RASSF1A group were measured by flow cytometry. D, MiR‐330‐3p mimics increased cell invasion, which was reversed by RASSF1A. The data are from one representative experiment among three that were performed identically and are expressed as the means ± SD. *P < 0.05 and **P < 0.01