Figure 5.

Circ_0078767 directly binds to miR‐330‐3p and suppresses miR‐330‐3p activity. A, Heat map: Among 20 circRNA, circ_0078767 was significantly lowly expressed in cervical cancer tissues compared with that in adjacent tissues. B, The estimated circ_0078767 binding sites for miR‐330‐3p. C, qRT‐PCR results showed that circ_0078767 expression in tumours was significantly higher than in adjacent tissues. D, qRT‐PCR results showed that circ_0078767 expression in non‐small‐cell lung cancer (NSCLC) cell lines was significantly lower than that in normal human lung epithelial cell lines. E, circ_0078767 expression was negatively correlated with miR‐330‐3p (R = 0.69, P = 0.001) and positively correlated with RASSF1A (R = 0.78, P = 0.001) in NSCLC tissues. F, miR‐330‐3p decreased the luciferase activity of the wild‐type circ_0078767 3'‐UTR. G, Anti‐AGO2 RIP was performed in the A549 cell line transfected with miR‐330‐3p mimics or negative control (NC), followed by qRT‐PCR to detect circ_0078767. The data are from one representative experiment among three that were performed identically and are expressed as the means ± SD. **P < 0.01