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. 2015 Oct 15;48(6):650–660. doi: 10.1111/cpr.12218

Figure 6.

Figure 6

Alizarin red staining and mineralization assay of hASC s induced by different BMP s in vitro . (a) After 21 and 28 days induction, positive results for alizarin red staining confirmed the osteoinductive effect of BMPs. However, there were no notable differences between the three BMPs. (b) After 21 and 28 days induction, mineralization assays demonstrated that hASC matrix calcifications were significantly enhanced by BMPs compared to PM and OM (P < 0.05). However, there were no significant differences between the three BMPs (P > 0.05). *P < 0.05 compared to PM, $ P < 0.05 compared to OM, & P < 0.05 compared to OM + 200 ng/ml BMP‐7. hASCs, human adipose‐derived stem cells; PM, proliferation medium; OM, osteogenic medium; BMP, bone morphogenetic protein.