Figure 1.

BIX 01294 does not alter myocardial function and phenotype. (a) Newborn rat cardiomyocytes were cultured in the absence or presence of BIX01294 and imaged on live video up to 9 days after initiation of culture in order to determine rates of myocyte beating. No significant difference was observed between the treated and non‐treated cultures at every time point. (#) Isoproterenol was added to both groups on day 6 for a positive inotropic effect. Beating data were compiled from four independent cultures for each time point. Parallel cultures of (b) non‐treated control and (c) BIX01294‐treated rat cardiomyocytes were also analysed for their sarcomeric architecture, as visualized by α‐actinin immunofluorescent staining. Note that BIX01294 did not noticeably affect the striated pattern of α‐actinin expression within individual myocytes. Scale bar = 20 μm.