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. 2016 Apr 24;49(3):373–385. doi: 10.1111/cpr.12255

Figure 4.

Figure 4

BIX 01294 treatment enhances BrdUrd incorporation by phase bright cells. Atrial tissue was exposed to BrdUrd for 48 h following a 7‐day incubation in the (a,b) absence or (c,d) presence of 8 μm BIX01294. Afterwards, phase bright cells were collected, cytospun onto glass slides, immunostained for BrdUrd incorporation and counterstained with DAPI to mark all cell nuclei. The corresponding BrdUrd and DAPI staining are showed side‐by‐side for individual fields of phase bright cells harvested from (a,b) non‐treated or (c,d) BIX01294‐treated tissue, respectively. Scale bar = 50 μm. (e) Calculation of per cent of BrdUrd‐positive phase bright cells in the absence or presence or various doses of BIX01294 for 2 (n = 8) and 7 days (n = 10). ANOVA analysis was used to analyse statistical significance of each BIX01294 dosage versus the control cultures. F(3,32) = 41.573; *P < 0.05; ***P < 0.001.