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. 2016 Apr 24;49(3):373–385. doi: 10.1111/cpr.12255

Figure 5.

Figure 5

BIX 01294 treatment enhances Ki67 expression by phase bright cells. Following the incubation of atrial tissue for 7 days in the (a,b) absence or (c,d) presence of 8 μm BIX01294, phase bright cells were harvested and cytospun onto glass slides. Afterwards, the cells were immunostained for Ki67 expression and counterstained with DAPI. Individual fields of phase bright cells harvested from (a,b) non‐treated or (c,d) BIX01294‐treated tissue are displayed with the corresponding Ki67 and DAPI staining showed in adjacent panels. Scale bar = 50 μm. (e) Tabulation of per cent of Ki67‐positive phase bright cells in the absence or presence of BIX01294. Statistical significance of increased staining within BIX01294‐treated versus control cultures was determined by the t‐test. ***P < 0.001 (n = 9).