Figure 6.

Expression of progenitor markers in atrial‐derived phase bright cells. Phase bright cells were collected from either (a,c,e,f) non‐treated control or (b,d,g,h) BIX01294‐treated atrial cultures, and immunofluorescently stained (grey) following (a–d) cytospinning onto glass slides or (e‐h) their attachment on culture plastic. Cultures were counterstained with DAPI to visualize nuclei (blue). Phase bright cells collected from control and treated cultures showed similar patterns and prevalence of expression for (a,b) CD90 and (c,d) Sca1, which are stem cell markers associated with CPCs. Differences were also not observed among phase bright cells obtained from (e,f) non‐treated or (g,h) BIX01294‐treated cultures, respectively, for the expression of cardiac progenitor marker Islet1. For this transcription marker, antibody and DAPI staining is shown independently for individual cellular fields, as indicated in the corresponding panels. Scale bar = 20 μm.