Figure 4.

Confocal microscopy of dual immunolabelling of mitochondria (green fluorescence) and actinic cytoskeleton (red fluorescence) in B50 cells. Compared to untreated controls (a), in cisPt‐treated cells (b), mitochondria clustered around the nucleus and formed dense masses in the cytoplasm. Control cells (a) revealed a filamentous actin skeleton; in (b) cisPt induced disruption of filamentous actin structures and assembly of depolymerized actin in peripheral regions of cytoplasm close to the cell membrane. DNA was counterstained with Hoechst 33258. Confocal microscopy: immunocytochemical detection of Bcl‐2 (green fluorescence) in control cells (c) and after cisPt treatment (d). After treatment with cisPt, Bcl‐2 protein levels increased significantly in the nucleus. The cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence).