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Biological evaluation in MCF‐7 cells for compounds 4 and 5. Cell cycle analysis (a) and pre‐G1 phase (b) in MCF‐7 cells untreated (DMSO, negative control), treated with SAHA (positive control) at 5 μm and with compounds 4 and 5 at 5 and 10 μm for 12, 24 and 48 h. All data expressed as means ± standard deviation (SD) of three independent experiments. Statistical significance P‐value: 0.001 except * and # marked 0.05.
