Figure 5.

Effect of iron deprivation on the level of Bcl‐2 protein in sensitive cells (human Raji, mouse 38C13) and in resistant cells (human HeLa, mouse EL4). Cells were incubated in iron‐free (–Fe) medium and cells of the control were incubated in transferrin (5 µg/ml) medium. The protein level was determined by indirect immunofluorescence analysis, employing specific monoclonal anti Bcl‐2 antibody Bcl‐2–100 (human cells) and specific monoclonal anti‐Bcl‐2 antibody 3F11 (mouse cells), after 48 h (human cells) or 24 h (mouse cells) of incubation. Stained cells were analysed by flow cytometry. The bold line represents staining with anti‐Bcl‐2 antibody and the fine line represents staining with relevant control non‐specific immunoglobulin. The data shown were obtained in one representative experiment of three independent experiments.