Figure 1.

  Functional annotation of total proteins secreted in ASC media and Nano RP LC–ESI–MS/MS analysis of TGB‐β1 and type 2 collagen proteins. (a) Proteins classified by biological processes. Values represent number of proteins classified into the indicated functional categories. (b) Nano RP LC–ESI–MS/MS analysis of peptide mixture of TGB‐β1 and type 4 collagen. (c) Total ion chromatogram of reverse‐phase LC separation of peptide mixture and MS spectrum of peptides eluted and detected at a dotted line of TGB‐β1 and type 4 collagen.