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. 2010 Apr 28;43(3):249–261. doi: 10.1111/j.1365-2184.2010.00680.x

Figure 3.

Figure 3

  Effects of cell density and concentration of ASC CM on chondrogenic potency in cultured ASCs and SPCs. (a) Western blot analysis of secreted type 2 collagen at various‐fold concentrations in ASC CM. (b) Cell density effects on chondrogenic activity in ASCs and SPCs. Toluidine blue‐positive cells enumerated after chondrogenic differentiation and expressed as percentage relative to total number of cells. (c) Effect of density of ASCs on chondrogenic activity. At various densities of cultured ASCs, different efficiencies of chondrogenic behaviour were evident. Cell density of 100% resulted in most efficient chondrogenic behaviour. (d–e) Effect of ASC CM concentration on chondrogenesis of ASCs (d) and SPCs (e) (*P < 0.05, **P < 0.01).