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ASC CM effectively induces chondrogenesis of cultured ASCs and SPCs in NOD/SCID mice. (a) Western blot and immunohistochemistry revealed ASC CM effectively promotes chondrogenesis of ASCs and SPCs in vivo in a CM concentration‐dependent manner (*P < 0.05, **P < 0.01). (b) Confirmation of chondrogenic activity of ASC CM combined ASCs in NOD/SCID mice by histological treatment with alizarin red, Masson’s trichrome, and van Gieson’s stain.
