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. 2016 Mar 10;49(2):207–218. doi: 10.1111/cpr.12246

Figure 1.

Figure 1

The expression of miR‐30a in clinical specimens and primary chondrocytes. (a) Chondrocyte micropellets from both healthy (normal) donors and donors with osteoarthritis (OA) were identified by hematoxylin‐eosin (HE), Alcian blue (AB) staining, and Col II immunohistochemistry. Scale bars: 100 μm. (b–c) The expression of miR‐30a in cartilage tissue (b) or primary chondrocytes (b) from healthy and OA donors was analyzed by real‐time PCR. Data were normalized to U6. (d–e) The expression of Sox9 mRNA (d) and protein (e) was analyzed by real‐time PCR and western blot, respectively. Data were normalized to β‐actin. All data were expressed as mean ± SD of three independent experiments. Normal, n = 7. OA, n = 11. *P < 0.05 versus normal.