Figure 1.

Sorting of ${\mathbf{P}}_{{\mathbf{75}}^{+}}$ EMSC s and ${\mathbf{P}}_{{\mathbf{75}}^{-}}$ EMSC s from ectomesenchymal cells isolated from the first branchial arch of the E11.5 SD rat. (a) Embryo from E11.5 SD rat, the first branchial arch (square frame) were dissected and served as the source of primary ectomesenchymal cells; (b) Primary ectomesenchymal cells; (c) Cells sorting by FCM, the ratio of ${\mathrm{P}}_{{75}^{+}}$ EMSCs was 24.5%; (d) ${\mathrm{P}}_{{75}^{+}}$ EMSCs after sorting; (e) ${\mathrm{P}}_{{75}^{-}}$ EMSCs after sorting; (f) Growth curve of ${\mathrm{P}}_{{75}^{+}}$ EMSCs and ${\mathrm{P}}_{{75}^{-}}$ EMSCs; *P < 0.05; bar:100 μm.