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. 2019 Apr 25;10:740. doi: 10.3389/fimmu.2019.00740

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Copyright © 2019 Cabral, da Silva Cândido, Monteiro, Lemos, Saitovitch, Noronha, Alves, Geraldo, Kalil, Cunha-Neto, Pinto Ferreira and Coelho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation assay showing increased levels of miR-885-5p in operational tolerance. Following the detection of higher levels of miR-885-5p in OT compared to chronic rejection in the global analysis, we performed a validation experiment by individual quantitative real-time PCR, using a specific primer for miR-885-5p, in a larger number of study subjects, using individual samples (OT n = 8, CR n = 12, HI n = 12; OT, operational tolerance; CR, chronic rejection; HI, Healthy individuals). Values are expressed as 2⁻ΔΔCt for miR-885-5p serum levels. For the statistical calculation we used the Kruskal-Wallis method followed by the Dunns post-test, and the results were considered significant if p < 0.05. ^**p = 0.0063 (OT × CR) and 0.0035 (OT × HI).