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. 2007 May 22;40(3):367–380. doi: 10.1111/j.1365-2184.2007.00439.x

Figure 2.

Figure 2

Immunocytochemical characterization of SSEA‐4 expression in stem cell lineages. Freshly isolated lineage negative (Panels a–d), CD133+ (Panels e–h) or CD34+ (Panels i–l) cells were stained with antihuman SSEA‐4 immunoglobulin G (IgG) (Panels b, f and j) or control IgG (Panels d, h and l) followed by fluorescein isothiocyanate (FITC)‐conjugated second antibodies (Panels b, d, f, h, j and l). Nuclei were then visualized by staining with 4′,6‐diamidino‐2‐phenylindole (DAPI) (Panels a, c, e, g, i and k). Fluorescence micrographs are representative of at least three independent isolations. Scale bars, 25 µm.