Figure 3.

  Cell cycle arrest in hAMSC after transfection with siDNER. (a) Representative diagrams of cell cycle distribution induced by siDNER or siControl‐transfected in hAMSC. Cell cycle distribution of hAMSC was analysed by flow cytometry as described in Materials and methods. The percentage of cells in each phase of the cell cycle was indicated as a bar graph. (b) Modulation of expression levels of CDK2, CDK4 and CCND1 upon siDNER transfection. Decrease in mRNA expression was detected in siDNER‐transfected group. RT‐PCR was conducted in triplicate for each transfectant. GAPDH was used as a loading control. (c) Densitometric analyses were obtained using the NIH ImageJ software for CDK2, CDK4 and CCND1 and the expression levels were normalized to GAPDH. The indicated values were expressed as the mean ± SD for three independent experiments.