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. 2011 Oct 20;44(6):537–549. doi: 10.1111/j.1365-2184.2011.00788.x

Figure 8.

Figure 8

  Real time PCR for osteoblast differentiation associated gene expression analysis. The graphs represent the relative fold difference in the gene expression on chitosan coated (chitosan) plates with respect to the untreated (UT) plates. The fold difference was calculated by the 2−ΔΔCT method. Cells on chitosan coated plates showed significantly high fold difference in the expression of OPN, IBSP and COL1A1, the three genes regulating respective proteins associated with calcium binding and bone matrix formation. ALPL showed a marked increase and a significantly high expression on day 14 and 21 of differentiation respectively. ON, a later stage and OCN, a final stage differentiation marker also showed significant fold difference on chitosan coated plates. Another key transcription factor OSX, regulating osteoblast differentiation, also showed a marked increase in expression on chitosan coated plates (*P < 0.05, **P < 0.01 and ***P < 0.001).