Table 1.

Levels of normalized Connexins mRNA in mouse lungs determined by quantitative RT‐PCR ^a

Groups	Difference in cycles between Cx43 and GAPDH ΔCT	Normalized Cx43 amount 2‐ΔΔCT
Cx43+/+	6.7 ± 0.2	1.2 ± 0.03
Cx43+/–	8.1 ± 0.7	0.5 ± 0.04*

^a Using reverse transcriptase, cDNA was synthesized from 500 µg of total RNA. Aliquots of cDNA were used as templates for real‐time PCR reactions containing primers and probes for Cx43, or primers and probe for GAPDH. Each reaction contained cDNA derived from 20 ng of total RNA. Eight different lungs from each group were analyzed, and four replicates of each reaction were performed by RT‐PCR.

^* P < 0.05, Tukey‐Kramer test.