Skip to main content
. 2015 Aug 13;48(5):517–531. doi: 10.1111/cpr.12205

Figure 2.

Figure 2

ANPC s derived from SVZ of HD mouse brain exhibit increased proliferation, migration and neurogenic differentiation compared with that of WT control. (a) Neurosphere assay. The size of neurospheres were determined after 8‐day culture in vitro (n = 70 neurospheres per group, ***P < 0.001). (b) YAC128 HD ANPCs had a much higher rate of growth compared with the WT control (n = 3, ***P < 0.001). (c,d) BrdU incorporation assay. The percentage of BrdU‐positive cells in YAC128 ANPCs was significantly higher than that in WT cells (n = 6, ***P < 0.001). Scale bar: 100 μm. (e) Neurospheres cultured on poly‐L‐ornithine dishes migrated outwards radially. Scale bar: 500 μm. (f) The radial migration distance of YAC128 HD neurospheres were significantly increased compared with the WT control after 12‐hour cultivation (n = 40 neurospheres per group, ***P < 0.001). (g) The percentage of neuronal cells (MAP2+) was quantified by normalizing the total MAP2+ cells to the total Hoechst33342+ cells after 21‐day differentiation (n = 3, **P < 0.01). (h) Quantification of the soma area of neurons derived from WT and YAC128 ANPCs (n = 50 neurons per group). (i) Neurite lengths of MAP2+ cells derived from WT and YAC128 ANPCs were measured. Independent t‐test showed a significant increase in neurite length in YAC128 neurons compared with WT neurons (n = 50 neurons per group, **P < 0.01). Data were shown as the mean ± SE.