Figure 1.

Effect of proteasome inhibition on endogenous PrP in NT-2 cells. Cells were treated without (control) or with MG132 at indicated concentrations for 12 hr. (A) Merged images of cells costained with anti-PrP antibody (red) and Lucifer yellow CH (green, lysosome staining) (Upper) or with anti-PrP (red) and anti-Hsc70 antibodies (green) (Lower). (B, Upper) MG132-treated cells were stained with anti-PrP antibody (red) and Lucifer yellow CH (green). The regions outlined by dashed boxes were merged and magnified (Right). (Lower) MG132-treated cells were stained with anti-PrP antibody (red) and anti-Hsc70 antibody (green).