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Physical state of PrP in NT-2 cells with or without proteasome inhibition. (A) Detergent cell lysates were sedimented, and PrP in the supernatant and pellet fractions was detected by immunoblot analysis with anti-PrP 3F4 monoclonal antibody. (B) Comigration of PrP from the pellet fraction with the recombinant mature fragment of PrP-(23–230), which lacks both N- and C-terminal signal sequences.
