Figure 4.

The physical state of PrP in transfected cells. (A) COS cells transfected with wild-type PrP were incubated with or without 1 μM epoxomicin for 16 hr. Detergent lysates were fractionated by centrifugation, and PrP in the supernatant (Sup) or pellet fractions was detected by immunoblot analysis using 3F4 antibody. (B) PrP in the pellet fraction was detected by immunoblot analysis with antibodies specific for the N-terminal (R24) or C-terminal (R20) regions of mature PrP as indicated. EndoH, PrP in the Endo H-digested pellet fraction was detected by 3F4 antibody.