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Physical state of PrPD177. COS cells were transfected as in Fig. 4. The activity of a cotransfected β-galactosidase construct (data not shown) demonstrated equivalent transfection efficiency. PrP was detected by 3F4 antibody. C, vector control; WT, PrP; M, PrPD177. (A, Left) Total PrP in lysates. (Right) PrP in Endo H-digested lysates. (B) PrP in supernatant and pellet fractions of cell lysates. The same blots were also probed with calnexin antibody.
