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. 2015 Feb 10;48(2):239–248. doi: 10.1111/cpr.12173

Figure 1.

Figure 1

Porphyromonas gingivalis lipopolysaccharides ( Pg‐ LPS) at 0.1 μg/ml accelerated and Pg‐ LPS at 10 μg/ml inhibited survival of bone marrow mesenchymal stem cells ( BMMSC s). (a, b) BMMSCs were treated with different concentrations (0, 0.1, 1 and 10 μg/ml) of Pg‐LPS. At 12, 24, 48 or 72 h after Pg‐LPS treatment, proliferation of BMMSCs was evaluated by Cell Counting Kit‐8 (a) or by using the trypan blue exclusion assay (b). (c) Apoptotic assay of BMMSCs was assessed by percentage of annexin V‐positive cells, by flow cytometry, 72 h after Pg‐LPS treatment. n = 6 in (a), n = 5 in (b) and n = 4 in (c). Results are representative of three independent experiments and are expressed as mean ± SD; statistical significance is shown as (*) P < 0.05, compared to BMMSCs without Pg‐LPS treatment. LPS, lipopolysaccharides from Porphyromonas gingivalis.