Figure 3.

  Destruction box at C‐terminus of HEC1 was required for its degradation. (a) Two mutants of HEC1, deleted and truncated HEC1, were subcloned into pCMV‐myc. Constructs derived from HEC1 used in this study are shown; number indicates amino acid position (Above). Expression of myc tagged two mutants and HEC1 verified by immunoblotting with anti‐myc antibody (Bottom). (b) Degradation of two mutated HEC1 by APC/C‐Cdh1. Deleted and truncated HEC1 were co‐transfected with hCdh1 into HEK293T cells and protein levels were detected by western blotting using anti‐myc antibodies.