Figure 3.

  Measurement data of colonies under the different experimental conditions (passage 1) of VUB01 cell line. Measurement data confirm visual observations regarding colony size under the different experimental conditions. In general, colonies formed with the fast protocol are smaller than those with the slow protocol, in the first passage. Colonies formed with TrypLE™ Express and Trypsin‐EDTA are the smallest, regardless of which adaptation protocol was applied. Those formed with Cell Dissociation Solution are of comparable size with the reference conditions, especially when the slow adaptation protocol was applied. Application of Cell Dissociation Solution and the fast protocol, resulted in smaller colonies. Colonies formed with Accutase were of small to intermediate size.