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Protocol overview
| Dissociative solution | Test protocol | Tools | |
|---|---|---|---|
| 1 | TrypLE™ Express | Slow protocol | Vigorous trituration |
| 2 | Trypsin‐EDTA | ||
| 3 | Cell Dissociation Solution | Occasional trituration | |
| 4 | Accutase™ | ||
| 5 | TrypLE™ Express | Fast protocol | Vigorous trituration |
| 6 | Trypsin‐EDTA | ||
| 7 | Cell Dissociation Solution | Occasional trituration | |
| 8 | Accutase™ | ||
| 9 | / | Manual mechanical passaging | Sharp glass cutting pipettes |
| 10 | Collagenase IV | Bead‐based passaging | Soda‐lime glass beads |
