Table 2.

 Overview of real‐time PCR EB analysis

	H1 cell line
	hESC			EB (differentiation)
	Manual mechanical passaging	Cell Diss Sol Slow prot	Cell Diss Sol Fast prot	Manual mechanical passaging	Cell Diss Sol Slow prot	Cell Diss Sol Fast prot
	Cond 9	Cond 3	Cond 7	Cond 9	Cond 3	Cond 7
Oct 4	+++	+++	+++	+	+	+
Nanog	+++	+++	+++	+	+	+
Hand1				+++	+++	+++
Col2A1				++	++	++
CD34				+++	+++	++
Runx2				++	++	++
Nestin				++	++	++
Sox9				++	+++	+++
Pax6				++	++	++
Cerberus				+	+	+
AFP				+++	+++	++
GATA4				++	++	++

	VUB01 cell line
	hESC			EB (differentiation)
	Manual mechanical passaging	Cell Diss Sol Slow prot	Cell Diss Sol Fast prot	Manual mechanical passaging	Cell Diss Sol Slow prot	Cell Diss Sol Fast prot
	Cond 9	Cond 3	Cond 7	Cond 9	Cond 3	Cond 7
Oct 4	+++	+++	+++	+	+	+
Nanog	+++	+++	+++	+	+	+
Hand1				+++	++	++
Col2A1				++	++	++
CD34				++	++	++
Runx2				++	++	++
Nestin				+	+	+
Sox9				++	++	++
Pax6				++	++	++
Cerberus				+	+	+
AFP				+++	+++	+++
GATA4				++	++	++