Figure 1.

Characterization of canine adipose mesenchymal stem cells. (A) Morphology of adherent CAMSCs, the cells present spindle‐like structures. (a, b) Cells were magnified 100‐fold. (c) Cells were magnified 200‐fold. (B) The growth curve of CAMSCs. After 8 days of culture, the cell number increased by 30‐fold. (C) PCR detection of cell surface and pluripotency markers. CAMSCs are positive for Sox2, Oct4, CD44 and CD166 and negative for CD21, CD34 and CD45, which share the same characteristics with mesenchymal stem cells