Figure 6.

BMP4‐induced CAMSC differentiate into male germ‐like cells. (a–c) Immunofluorescence staining to test germ cell markers. (a) CMSCs‐EB were induced with 12.5 ng/mL BMP4. After 7 days, the expression level of PLZF was distinctly higher than in the control group. (b) Detection of DMRT1 protein expression level by immunofluorescence staining. (c) Detection of VASA protein expression level by immunofluorescence staining. (d) qRT‐PCR analysis of mRNA expression levels of PLZF, Dmrt1 and VASA in the induced group was significantly higher than those of the control group