Figure 4.

MiR‐200a is involved in regulation of growth hormone in the Keap1/Nrf2 pathway. Murine bone marrow‐derived dendritic cells (BMDCs) were treated with GH for 24 h and miR‐200a expression was then detected by real‐time PCR (a). BMDCs were transfected with an miR‐200a mimic or negative control (NC) after treatment with GH; protein expressions of Keap1 (b), Nrf2 (c), and Nrf2 targets (d) in BMDCs were measured by western blotting with α‐tubulin as internal reference protein; *versus control, P < 0.05.