Testis histological phenotype and
PLZF
expression pattern in the dairy goat. (a) Control groups for immunohistochemical staining of three age groups (a*, d*, g*); HE staining of the three different age groups (c*, f*, i*); PLZF immunohistochemical staining of the testicular tissues of the three different age groups (b*, e*, h*). Bar = 65.34 μm (a*–i*). (b) Percentage of PLZF‐positive cells per seminiferous tubule cross‐section. *P < 0.05, **P < 0.01. (c) PLZF expression at three different ages of testicular tissue was analysed by RT‐PCR. (d) Relative expression values of PLZF at the three different ages (measured by ImageJ). **P < 0.01.