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BrdU incorporation assay. (a) Proliferation of mGSCs was determined by BrdU incorporation assay in PLZF overexpression. (a*–c*) Cells treated with pPLZF‐IRES2‐EGFP; (d*–f*) Cells treated with pIRES2‐EGFP; (g*–i*) Blank group, transfected, but with no plasmid. (b) Quantification of (a). **P < 0.01.
