Skip to main content
. 2009 Mar 13;42(2):123–131. doi: 10.1111/j.1365-2184.2009.00589.x

Figure 2.

Figure 2

Characteristics of the adult spermatogonial stem cell (SSC) line. (a) Characteristic example of Oct4 expression in an SSC clump. Green indicates Oct4 immunofluorescence, blue indicates DAPI immunofluorescence. (b) Most SSCs were negative for c‐kit (99 ± 1%), the red channel represents c‐kit immunofluorescence and the blue channel represents DAPI immunofluorescence. (c, d) Cytogenetic analysis by DAPI staining showed that these SSCs had a normal karyotype (40, XY). (e) Flow cytometry analysis of SSCs, DNA content was measured to show cell ploidy. Adult mouse testes were used as a reference (data not shown). (f) TRAP telomerase activity assay showed that the cells had high telomerase activity. M, DNA marker; lane 1, F9 cells (positive control); lane 2, SSCs; lane 3, STO (negative control). Data shown are representative of four experiments. Scale bars: 10 µm in (a), 25 µm in (b) and 6 µm in (c).