Figure 3.

Progenies generated from transplantation of adult spermatogonial stem cells (SSC) after cryopreservation. (a–c) Testis histology of busulphan‐treated recipient mice. (a) Control testes without cell transplantation (note the absence of spermatogenesis). (b) Section of the recipient testes at 6 weeks after transplantation of adult SSCs. Note active spermatogenesis and long spermatids in seminiferous tubules (arrow). (c) Section of recipient testis 12 weeks after transplantation treatment. Sperms are seen (arrow). (d) Progenies from a C57BL/6 × CD‐1 F₁ recipient mouse transplanted with C57BL/6 adult SSCs that were passaged more than 40 times and cryopreserved for 1 week, and their mother. The mother is a pure‐blood CD‐1 mouse with pure white coat, and all the offspring are black. (e) ISSR DNA fingerprinting revealed kinship of these mice. M, marker; lane 1, mating female; lane 2, recipient male; lane 3, SSC line; lanes 4–9, offspring. Discriminating bands are marked with arrows. Data shown are representative of three experiments. Scale bars: 100 µm in (a), 25 µm in (b) and 40 µm in (c).