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. 2007 Jan 15;40(1):91–105. doi: 10.1111/j.1365-2184.2007.00422.x

Figure 3.

Figure 3

Morphology and cell population growth properties of spontaneously immortalized pig fibroblasts and those of the derived hybrid cells. (a) Morphology of spontaneously immortalized pig fibroblasts AG12077‐IM. (b) Fusion of AG12077‐IM cells with F7 cells to produce a mass culture, with immortal fibroblast‐like morphology when selected in HAT medium (note: no growth activation). (c) Morphology of immortal AG12077‐IM‐hTERT after introduction of hTERT. (d) Fusion of AG12077‐IM‐hTERT cells with F7 cells produced cells similar to immortal pig fibroblasts when fused cells were grown in HAT medium (note: no growth activation). (e) Morphology of the immortal AG12077‐IM cells after artificially induced ageing. (f) Fusion of aged AG12077‐IM cells with F7 cells occasionally produced colonies of slow‐growing hybrids in the background of ageing fibroblasts. (g) Tightly packed, slow‐growing hybrids resulted from the fusion of AG12077‐IM‐hTERT cells with F7 cells, selected in DMEM containing G418 and puromycin. (h) Karyotype of the immortal cell line AG12077‐IM showing monosomy of chromosomes 4, 5, 9, 10, 11 and nullisomy of chromosome 12, in addition to three marker chromosomes (indicated by arrows). Βar = 100 µm.