Table 1.

Summary of results of a variety of combinatorial cell fusion experiments involving mouse and pig fibroblasts

Mouse parental cell lines	Pig fibroblast strains/lines	Selective system used for hybrid isolation	Genomic composition of most metaphase cells	Growth activation in selected cells
F7 a	AG08114 c	HAT + G418	2 : 1 pig–mouse hybrid	Yes
F7 a	AG12077 c	HAT + G418	2 : 1 pig–mouse hybrid	Yes
F7 a	AG08114 c	HAT only	2 : 1 pig–mouse hybrid	Yes
F7 a	AG12077 c	HAT only	2 : 1 pig–mouse hybrid	Yes
GM05267 b	AG08114 c	HAT only	2 : 1 pig–mouse hybrid	Yes
GM05267 b	AG12077 c	HAT only	2 : 1 pig–mouse hybrid	Yes
F7 a	AG12077‐IM d	HAT only	Pig fibroblast	No
F7 a	AG12077‐IM d	HAT + G418	2 : 1 and 1 : 1 hybrids	No
F7 a	AG12077‐IM‐Aged d	HAT only	Pig fibroblast	No
F7 a	AG12077‐IM‐Aged d	HAT + G418	2 : 1 and 1 : 1 hybrids	No
F7 a	AG12077‐IM + hTERT d	HAT + G418	2 : 1 and 1 : 1 hybrids	No

^a cells of this mouse cell line had monosomy of chromosomes 6, 7, 8, 9, 12, 17 and 18.

^b cells of this mouse cell line had monosomy of chromosomes 7, 8, 9, 12, 17 and 18.

^c most of the karyotypes of the porcine cell strain had normal female pig karyotype (38, XX).

^d average karyotype of cells of this porcine cell line was 35–36, XX, −4, −5, −9, −10, −11, −12, −12, +3–4mar.