Table 1.
Mouse parental cell lines | Pig fibroblast strains/lines | Selective system used for hybrid isolation | Genomic composition of most metaphase cells | Growth activation in selected cells |
---|---|---|---|---|
F7 a | AG08114 c | HAT + G418 | 2 : 1 pig–mouse hybrid | Yes |
F7 a | AG12077 c | HAT + G418 | 2 : 1 pig–mouse hybrid | Yes |
F7 a | AG08114 c | HAT only | 2 : 1 pig–mouse hybrid | Yes |
F7 a | AG12077 c | HAT only | 2 : 1 pig–mouse hybrid | Yes |
GM05267 b | AG08114 c | HAT only | 2 : 1 pig–mouse hybrid | Yes |
GM05267 b | AG12077 c | HAT only | 2 : 1 pig–mouse hybrid | Yes |
F7 a | AG12077‐IM d | HAT only | Pig fibroblast | No |
F7 a | AG12077‐IM d | HAT + G418 | 2 : 1 and 1 : 1 hybrids | No |
F7 a | AG12077‐IM‐Aged d | HAT only | Pig fibroblast | No |
F7 a | AG12077‐IM‐Aged d | HAT + G418 | 2 : 1 and 1 : 1 hybrids | No |
F7 a | AG12077‐IM + hTERT d | HAT + G418 | 2 : 1 and 1 : 1 hybrids | No |
cells of this mouse cell line had monosomy of chromosomes 6, 7, 8, 9, 12, 17 and 18.
cells of this mouse cell line had monosomy of chromosomes 7, 8, 9, 12, 17 and 18.
most of the karyotypes of the porcine cell strain had normal female pig karyotype (38, XX).
average karyotype of cells of this porcine cell line was 35–36, XX, −4, −5, −9, −10, −11, −12, −12, +3–4mar.