Figure 1.
Growth features of cultured HTR‐8 SV/neo trophoblast cell line shown for various culture nutrients, in absence (a) or presence (b) of FBS and placental extract proteins (PEPs) including PEP‐A (c), PEP‐B (d) and PEP‐C (e) at 72 h. Cell viabilities of HTR‐8 SV/neo trophoblast cells with 0.5 μg concentrations of PEP‐A and PEP‐B were approximately 70% of those in conventional culture using FBS. But, growth of HTR‐8 SV/neo trophoblast cells using PEP‐C is similar to that in absence of FBS (×100 original magnification).