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. 2010 Aug 30;43(5):435–444. doi: 10.1111/j.1365-2184.2010.00693.x

Table 1.

 Sequence of primers used for RT‐PCR and length of fragments

Genes Sequence T m (°C) Size (bp)
OCT4 F: 5′‐ACA CTC GGA CCA CGT CTT TC‐3′ 54 300
R: 5′‐CGT TCT CTT TGG AAA GGT GTT C‐3′
Nanog F: 5′‐TTC TTG ACT GGG ACC TTG TC‐3′ 54 300
R: 5′‐GCT TGC CTT GCT TTG AAG CA‐3′
Sox2 F: 5′‐GGG CAG CGT GTA CTT ATC CT‐3′ 52 200
R: 5′‐AGA ACC CCA AGA TGC ACA AC‐3′
NF‐68 F: 5′‐GAG TGA AAT GGC ACG ATA CCT A‐3′ 58 500
R: 5′‐TTT CCT CTC CTT CTT CTT CAC CTT C‐3′
Cardiac F: 5′‐GGA GTT ATG GTG GGT ATG GGT C‐3′ 58 500
R: 5′‐AGT GGT GAC AAA GGA GTA GCC A‐3′
hAFP F: 5′‐AGC TTG GTG GAT GAA AC‐3′ 50 200
R: 5′‐TCC AAC AGG CCT GAG AAA TC‐3′
HLA‐G F: 5′‐GCG GCT ACT ACA ACC AGA GC‐3′ 58 550
R: 5′‐GCA CAT GGC ACG TGT ATC TC‐3′
TERT F: 5′‐GAG CTG ACG TGG AAG ATG AG‐3′ 55 300
R: 5′‐CTT CAA GTG CTG TCT GAT TCC AAT G‐3′
β‐actin F: 5′‐TCC TTC TGC ATC CTG TCA GCA‐3′ 58 300
R: 5′‐CAG GAG ATG GCC ACT GCC GCA‐3′
HNF1α F: 5′‐AC ACC ACT CTG GCA GCC ACA CT 58 114
R: 5′‐CGG TGG GTA CAT TGG TGA CAG AAC
HNF4α F: 5′‐CTG CTC GGA GCC ACC AAG AGA TCC ATG‐3′ 55 370
R: 5′‐ATC ATC TGC CAC GTG ATG CTC TGC A‐3′
CXCR4 F: 5′‐ACG TCA GTG AGG CAG ATG‐3′ 58 202
R: 5′‐GAT GAC TGT GGT CTT GAG‐3′
Albumin F: 5′‐CCC CAA GTG TCA ACT CCA AC‐3′ 54 450
R: 5′‐CTC CTT ATC GTC AGC CTT GC‐3′
TAT F: 5′‐AAC GAT GTG GAG TTC ACG G‐3′ 59 288
R: 5′‐GAC ACA TCC TCA GGA GAA TGG‐3′
28s rRNA F: 5′‐TTG AAA ATC CGG GGG AGA G‐3′ 52 100
R: 5′‐ACA TTG TTC CAA CAT GCC AG‐3′