Table 1.
Sequence of primers used for RT‐PCR and length of fragments
Genes | Sequence | T m (°C) | Size (bp) |
---|---|---|---|
OCT4 | F: 5′‐ACA CTC GGA CCA CGT CTT TC‐3′ | 54 | 300 |
R: 5′‐CGT TCT CTT TGG AAA GGT GTT C‐3′ | |||
Nanog | F: 5′‐TTC TTG ACT GGG ACC TTG TC‐3′ | 54 | 300 |
R: 5′‐GCT TGC CTT GCT TTG AAG CA‐3′ | |||
Sox2 | F: 5′‐GGG CAG CGT GTA CTT ATC CT‐3′ | 52 | 200 |
R: 5′‐AGA ACC CCA AGA TGC ACA AC‐3′ | |||
NF‐68 | F: 5′‐GAG TGA AAT GGC ACG ATA CCT A‐3′ | 58 | 500 |
R: 5′‐TTT CCT CTC CTT CTT CTT CAC CTT C‐3′ | |||
Cardiac | F: 5′‐GGA GTT ATG GTG GGT ATG GGT C‐3′ | 58 | 500 |
R: 5′‐AGT GGT GAC AAA GGA GTA GCC A‐3′ | |||
hAFP | F: 5′‐AGC TTG GTG GAT GAA AC‐3′ | 50 | 200 |
R: 5′‐TCC AAC AGG CCT GAG AAA TC‐3′ | |||
HLA‐G | F: 5′‐GCG GCT ACT ACA ACC AGA GC‐3′ | 58 | 550 |
R: 5′‐GCA CAT GGC ACG TGT ATC TC‐3′ | |||
TERT | F: 5′‐GAG CTG ACG TGG AAG ATG AG‐3′ | 55 | 300 |
R: 5′‐CTT CAA GTG CTG TCT GAT TCC AAT G‐3′ | |||
β‐actin | F: 5′‐TCC TTC TGC ATC CTG TCA GCA‐3′ | 58 | 300 |
R: 5′‐CAG GAG ATG GCC ACT GCC GCA‐3′ | |||
HNF1α | F: 5′‐AC ACC ACT CTG GCA GCC ACA CT | 58 | 114 |
R: 5′‐CGG TGG GTA CAT TGG TGA CAG AAC | |||
HNF4α | F: 5′‐CTG CTC GGA GCC ACC AAG AGA TCC ATG‐3′ | 55 | 370 |
R: 5′‐ATC ATC TGC CAC GTG ATG CTC TGC A‐3′ | |||
CXCR4 | F: 5′‐ACG TCA GTG AGG CAG ATG‐3′ | 58 | 202 |
R: 5′‐GAT GAC TGT GGT CTT GAG‐3′ | |||
Albumin | F: 5′‐CCC CAA GTG TCA ACT CCA AC‐3′ | 54 | 450 |
R: 5′‐CTC CTT ATC GTC AGC CTT GC‐3′ | |||
TAT | F: 5′‐AAC GAT GTG GAG TTC ACG G‐3′ | 59 | 288 |
R: 5′‐GAC ACA TCC TCA GGA GAA TGG‐3′ | |||
28s rRNA | F: 5′‐TTG AAA ATC CGG GGG AGA G‐3′ | 52 | 100 |
R: 5′‐ACA TTG TTC CAA CAT GCC AG‐3′ |