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Effect of PDE 9 inhibitor ( BAY 73‐6691) on c GMP levels in MCF ‐7 and MDA ‐ MB ‐468 cells. Cells were treated with 200 μm BAY 73‐6691 over a time course. Measurement of cGMP was performed using a colorimetric competitive ELISA kit. Each point, mean ± SD of three experiments. Each experiment was repeated three times. *P < 0.05; **P < 0.01 compared to untreated control groups.
